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* Edge enhancers
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* Despeckle
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Provide examples of Do's and Don'ts....
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## Specific guidelines
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* For primary antibody and secondary antibody combinations, controls such as a non-immune antibody, omission of the primary antibody, and absence of antigen may be necessary to demonstrate specificity. [JBC]
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* Do not prepare images in PowerPoint or save them in JPEG format.
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* If you are using the same control in two figures clearly state this in the figure legend.
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* Do not make your image too small (postage stamp size) such that the detail cannot be seen.
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* Do not put too many images on one slide or figure.
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* When adding scale bars, if do not add scaling to image, then this informaion must be place in the legend. Where all images have the same scale bar and hence scaling in one figure, the the scale bar can be added to one image in the figure only. But it must be stated in the legend that the scaling/scale bar is the same in all images in the figure.
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* Ensure that your images are not stretched or misaligned when forming a group of figures. Use gridlines and snap to gridline to assist you in grouping images in a figure.
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* Label your images appropriately with letters, numbers, scale bars, arrow, arrowheads, large and small etc..to identify specific features highlighted in the description/legend.
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## Intensity measurements
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Images gathered at different times or from different locations should not be combined into a single image, unless it is stated that the resultant image is a product of time-averaged data or a time-lapse sequence. If juxtaposing images is essential, the borders should be clearly demarcated in the figure and described in the legend.
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Additional information: NB. There are a myriad of ways to present you data from an image eg. overlay, line profile, thresholded images, co-localisation scatterplots, gallery of z- or t-series, montage, stitched image, maximum intensity projection, 3-D orthogonal, volume view. Think of the ways to best illustrate/display your data. Provide examples.
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[Nature]: https://www.nature.com/authors/policies/image.html
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[ORI]: https://ori.hhs.gov/education/products/RIandImages/guidelines/list.html
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