figure/Guidelines.md

@@ -46,37 +46,37 @@ Filters that treat parts of image differently
 
 * For primary antibody and secondary antibody combinations, controls such as a non-immune antibody, omission of the primary antibody, and absence of antigen may be necessary to demonstrate specificity. [JBC]
 * Do not prepare images in PowerPoint or save them in JPEG format.
-* If you are using the same the control in two figures clearly state this in the figure legend.
+* If you are using the same control in two figures clearly state this in the figure legend.
 
 
 ## Intensity measurements
 
 When comparing intensity measurements:
 
-* All images must be taken under identical settings (lamp power, filters, exposure etc)
-* All images must be processed the same way
+* All images must be taken under identical settings (lamp or laser power, filters, exposure, gain, offset, pinhole size, optical Z-slice etc)
+* All images must be processed the same way and reported as such
 * Ideally images acquired in the same session.
 
 ## Reporting
 
-* type of microscope, 
-* objective lenses, cameras
+* Name and type of microscope, 
+* objective lenses, name and type, magnification, numerical aperture (NA), dry or immersion lens
 * camera / detectors
-* fluorophore excitation and emission wavelengths or ranges
-* details of the light sources, filters and beamsplitters 
+* fluorophore excitation and emission wavelengths or ranges ie. short pass, long pass or bandwidth
+* details of the light sources, ND filters and/or beamsplitters 
 * Name of the acquisition software program and version number
 * Name of the processing software program and version number
-* Name of tool / plugins within that program and their mportant settings.
+* Name of tool / plugins within that program and their important settings.
 
-#### From [Nature]:
+#### Adapted from [Nature]:
 A single Supplementary Methods file (or part of a larger Methods section) titled "Equipment and Settings" should list for each image: 
 
-* acquisition information, 
+* all acquisition information (see above), 
 * time and space resolution data (xyzt and pixel dimensions)
 * image bit depth
-* experimental conditions such as temperature and imaging medium;
+* experimental conditions such as temperature, gas requirement (if any) and imaging medium;
 *  fluorochromes excitation and emission wavelengths or ranges 
-*  filters, dichroic beamsplitters.
+*  ND filters, dichroic beamsplitters.
 
 Images gathered at different times or from different locations should not be combined into a single image, unless it is stated that the resultant image is a product of time-averaged data or a time-lapse sequence. If juxtaposing images is essential, the borders should be clearly demarcated in the figure and described in the legend.