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# Setup for live cell
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## 1. Turn on the main CO2 supply
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## 1. Turn on the main CO$`_2`$ supply
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The carbon dioxide is supplied from the tank opposite the Nikon Ti room. When CO$`_2`$ is not being used the tanks should all be off and the main gauge reading 0 kPa.
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Open the active tank by turning the tap on the top of the tank. The main gauge should now read about 400 kPa.
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