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# Experiment Design (ND Acquisition)
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The ND acquisition tab let you combine the optical configurations you have saved into complex experiments.
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## Save Location
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![](media/ND-aq1.png)
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* Path - location to save the file. It is convenient to save directly to mif_home.
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* File name - This is automatically incremented each time the acquisition is run.
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## Timelapse
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![](media/time-points-detail.png)
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1. Click on `Time` tab
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2. Add timing loops
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You need to ensure that there is enough time to complete the combining multiple channels, Z-stacks and fields of view.
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## XY points
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### Manually adding points
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![](media/XY-points-detail.png)
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1. Check the `Include Z` box (this saves the focal plane)
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2. Move the stage with to new position.
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1. Click the `+` to add the current stage position.
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2. Points can deactivated by unchecking the boxes.
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3. Points can reordered by clicking the blue arrows
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Tips:
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* Click on the points to visit the location (`Move Stage to Selected Point` must be checked)
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* Select `Split Multipoints` under the `Advanced` if you a large numbers of point. This makes importing and analysis easier.
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* Update the XY position for a point by clicking the `->`
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* Update the Z position for a point by clicking the `<-`
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* PFS offsets can saved for each point.
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* `Optimize` will reorder you points so that they are visited efficiently.
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## Z-stack
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![](media/Z-stack-detail.png)
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## Multiple channels
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![](media/multi_channel-detail.png)
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1. Click `+` to add a channel
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2. Select the Optical Configuration from the drop down
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3. Channels can deactivated by unchecking them.
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4. Channels can reordered using the blue arrows
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