Nikon-Ti/Koehler/Setting-up-Kohler-Illumination.md

+# Setting up Koehler Illumination
+
+![](condenser-markup.jpg)
+
+1. Select the lens
+2. Click on the Bright Field OC (or set the condenser to `A`)
+3. Make sure the polariser is out
+4. Focus on the sample, the edge of the slide or coverslip is easy place to start
+5. Close the Condenser Aperture Diaphragm, you should see the octagon formed by the blades
+6. Raise and lower the condenser so that the blades are in focus
+7. Adjust the centering screws so that the octagon in the centre of the image
+8. Open the Condenser Aperture Diaphragm  so the blades are just past the camera field of view
+9. Remove eye piece and adjust the Condenser Aperture so that 2/3 of pupil is filled. 
+
+## For Phase Contrast
+
+1. Click on the lens OC (or set the condenser to correct Phase setting)
+2. Remove the eye piece and 
+
+## For DIC
+
+1. Insert the Polariser (or set the condenser to 'A')
+2. Click on the lens OC
+3. Adjust the angle with silver rod to give the best contrast. 
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