... | @@ -4,60 +4,20 @@ The Microbial Imaging Facility (MIF) is located within PC2 laboratories which al |
... | @@ -4,60 +4,20 @@ The Microbial Imaging Facility (MIF) is located within PC2 laboratories which al |
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Available Instruments:
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Available Instruments:
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#### Nikon Ti inverted epifluorescent microscope
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#### Nikon Ti inverted epifluorescent microscope
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Pphase contrast and DIC objectives, CCD and EMCCD cameras and live cell imaging capability (humidified environmental chamber with temperature and CO2 (control) with Perfect Focus System ™.
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Filter Cubes:
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* mCherry (HC Red),
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* GFP B, YFP HQ, Texas Red, Cy5.5, DAPI, UV-2A, GFP HQ, TRITC
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#### Nikon A1 laser scanning confocal microscope
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#### Nikon A1 laser scanning confocal microscope
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with MadCity ultra-fast piezo Z-drive, filter and spectral detection, phase contrast and DIC objectives, galvano and resonance scanners and live cell imaging capability (humidified environmental chamber with temperature and CO2 (control) with Perfect Focus System ™
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Lasers: 404, 488, 561, 637 nm
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Emission: blue (425-475 nm), green (500 - 550 nm), red (570-620 nm), far red (662-737 nm)
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#### DeltaVision personalDV
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#### DeltaVision personalDV
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high resolution imaging system (inverted epifluorescent deconvolution microscope)
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Filter Cubes: DAPI, TRITC, FITC, Cy5
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#### DeltaVision personalDV with plate reader
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#### DeltaVision personalDV with plate reader
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high resolution imaging system (inverted epifluorescent deconvolution microscope) fitted with a WeatherStation environmental control chamber.
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Light sources: 405, 488, 514 and 593 nm lasers and LED-illuminated DIC
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Emission Filters: DAPI (405 EX / 419-465 EM), CFP (405 EX / 464.5-499.5 EM), Alexa488, EGFP (488 EX / 500-550 EM), YFP (514 EX / 500-507, 523.8-550 EM), Alexa594 (592.5 EX / 608-648 EM), mCherry (592.5 nm EX / 602.5-655.5 EM)
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#### BD LSRII analytical flow cytometer
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#### BD LSRII analytical flow cytometer
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with 4 lasers and 11 detection filters
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Filter Cubes - Standard set: DAPI, TRITC, FITC, Cy5
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Filter Cubes - Live cell set: CFP, YFP, GFP, mcherry
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##### [DeltaVision OMX Blaze](DV_OMX_SR/DeltaVision OMX SR)
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##### [DeltaVision OMX Blaze](DV_OMX_SR/DeltaVision OMX SR)
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for multi-wavelength simultaneous fast structured illumination imaging or fast simultaneous multi-wavelength high resolution deconvolution live cell imaging, with heated stage.
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Light sources: 405, 488, 514 and 593 nm lasers and LED-illuminated DIC
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Emission Filters: DAPI (405 EX / 419-465 EM), CFP (405 EX / 464.5-499.5 EM), Alexa488, EGFP (488 EX / 500-550 EM), YFP (514 EX / 500-507, 523.8-550 EM), Alexa594 (592.5 EX / 608-648 EM), mCherry (592.5 nm EX / 602.5-655.5 EM)
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#### BD LSRII analytical flow cytometer
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#### BD LSRII analytical flow cytometer
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with 4 lasers and 11 detection filters -
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Lasers: 325, 405, 488, 635 nm
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Emission Filters: Indo1 (blue), DAPI, Hoechst 33258, Indo-1 (Violet), FRET-YFP, Pacific orange, Pacific blue, CFP, PE-Cy5, PerCP, PerCP-Cy5.5, PE, PE-Cy7, PE-Texas Red, Alexa488, FITC, GFP, APC-Cy7, APC, Alexa700
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#### Advanced computing capabilities
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Image analysis software: NIS Elements, Bitplane IMARIS, SIS AnalySIS, ImageJ
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Flow Cytometry analysis software: Diva, FCAP Array, FlowJo, Modfit and CellQuest Pro
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Other software: Adobe Photoshop, Graphpad PRISM, Accelrys MacVector
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Additional computing resources are available within the Advanced Computing Facility (ACF) of MIF. The ACF is located on the same floor as the MIF outside of the wet labs and is designed for offline image and data analysis. |
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#### Software |
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