| ... | ... | @@ -5,11 +5,28 @@ If the lenses have been setup to be parfocal then if focus is found on one objec |
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The focus was on histological slide for each lense and Z-position recorded
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| Lens | Z |
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|----------|------|
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| 20X |
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| 40X ELWD |
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| 40X |
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| 60X |
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| 100X |
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| 100X | |
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| Lens | Z / um |
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|----------|---------|
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| 20X | 2033.75 |
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| 40X ELWD | 3304.38 |
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| 40X | 5265.08 |
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| 60X | 2005.85 |
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| 100X | 2061.97 |
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| 100X | 2055.35 |
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Following the instructions from page 84 the Nikon Eclipse Ti RCP manual the Auto-Link Function (ALF) was turned off and the ALF memory for each lens set at the focal points.
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With ALF off when the lens is changed the lens is escaped to 500um, the turret rotated, and the lens is return to intial position.
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1. ALF Off on the RCP
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Initial Focus with the 40X Z=5279
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a. Change to 60X with side of Ti Zf=5290
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b. Change from RCP Zf=5290
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c. Change from Software TiPad Z=5290, Zf=2000
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2. ALF On:
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a. 500, 5290, 2000
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