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# Setting up Koehler Illumination
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![](condenser-markup.jpg)
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1. Select the lens
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2. Click on the Bright Field OC (or set the condenser to `A`)
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3. Make sure the polariser is out
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4. Focus on the sample, the edge of the slide or coverslip is easy place to start
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5. Close the Condenser Aperture Diaphragm, you should see the octagon formed by the blades
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6. Raise and lower the condenser so that the blades are in focus
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7. Adjust the centering screws so that the octagon in the centre of the image
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8. Open the Condenser Aperture Diaphragm so the blades are just past the camera field of view
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9. Remove eye piece and adjust the Condenser Aperture so that 2/3 of pupil is filled.
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## For Phase Contrast
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1. Click on the lens OC (or set the condenser to correct Phase setting)
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2. Remove the eye piece and
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## For DIC
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1. Insert the Polariser (or set the condenser to 'A')
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2. Click on the lens OC
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3. Adjust the angle with silver rod to give the best contrast. |
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